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!pip install bottle


    

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!pip install biopython


    

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!apt-get install ncbi-blast+


    

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!wget https://bin.equinox.io/c/4VmDzA7iaHb/ngrok-stable-linux-amd64.zip


    

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!unzip ngrok-stable-linux-amd64.zip


    

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!wget https://github.com/Serulab/Py4Bio/archive/master.zip


    

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!unzip master.zip


    

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!cp -r Py4Bio-master/code/ch16/* ./


    

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!sed -i "s/port=8080/port=8888/" ./vector.py


    

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!sed -i "s%/home/sbassi/opt/ncbi-blast-2.6.0+/bin/blastn%/usr/bin/blastn%g" ./vector.py


    

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get_ipython().system_raw('./ngrok http 8888 &')


    

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%%sh
curl -s http://localhost:4040/api/tunnels | python -c "import sys, json; print(json.load(sys.stdin)['tunnels'][0]['public_url'])"


    

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!rm ./bottle.py


    

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!python ./vector.py


    

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